Holders Cefn Albion have landed a home draw against Lex in the second round of the 2019-20 FAW Trophy.
Welsh National League newcomers Halkyn and Flint Mountain will be looking to pull off a giant-killing when they host Denbigh Town, current co-leaders of Welsh Alliance Division One.
Newly-formed Chirk Town of the North East Wales League have been paired with Welsh National League Premier Division opponents in Llanuwchllyn – another David v Goliath encounter.
In the North West, new club Bangor 1876 will have a big scalp in their sights when the Gwynedd League outfit entertain Welsh Alliance Division Two side Pwllheli.
Llanfairpwll of the Anglesey League will aim to achieve a major shock when they welcome Llandudno Junction of the Welsh Alliance to Maes Eilian.
All ties will take place on September 14.
FAW Trophy round two draw
(Ties to be played September 14)
Acton v Mynydd Isa Spartans
Brickfield Rangers v Rhydymwyn
Brymbo FC v Llandyrnog United
Cefn Albion v Lex
Chirk AAA v Queen’s Park
Chirk Town v Llanuwchllyn
Coedpoeth United v Prestatyn Sports
Greenfield v Penycae
Hawarden Rangers v Holywell Town
Halkyn & Flint Mountain v Denbigh Town
Llangollen Town v Cefn Mawr Rangers
Overton Recreation v New Brighton Villa
Plas Madoc v Llay Welfare
Rhos Aelwyd v Castell Alun Colts
Rhostyllen v Rhosllanerchrugog
Saltney Town v Mold Alexandra
Amlwch Town v Llandudno Athletic
Bangor 1876 v Pwllheli
Barmouth & Dyffryn v Gwalchmai
Bodedern Athletic v Glantraeth
Gaerwen v Glan Conwy
Holyhead Hotspur v Aberffraw
Llanberis v Waunfawr
Llanfairpwll v Llandudno Junction
Llannefydd v Holyhead Town
Llanrwst United v Kinmel Bay
Mynydd Llandegai v Menai Bridge Tigers
Nantlle Vale v Blaenau Amateurs
Penmaenmawr Phoenix v Nefyn United
St Asaph City v Llandudno Albion
Y Felinheli v Llanrug United